Close any application that is using this file, and then try again. ‘The file C:\users\….username.ost is in use and cannot be accessed. However, sometimes due to a problem with OST file, an Outlook user may experience errors such as, When the server comes back online, all changes made in the local mailbox cache (.OST) are synchronized with the mailbox on the server. This ‘cached mode’ allows users to work offline on Outlook and improves Outlook performance. In Microsoft Exchange, if Cached Exchange Mode is ON, a replica of mailboxes residing on the server is created on the local machine with. However, there are a few troubleshooting methods that you may apply to fix the Outlook error. This can be resolved by converting the faulty OST file to PST format with Stellar Converter for OST. Summary: OST is an Outlook data file and problems with OST file often results in errors such as ‘The file xxx.ost is in use and cannot be accessed’.
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